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Livraison de gènes au cerveau de rat postnatal par des non-ventriculaire injection Plasmide et électroporation
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Livraison de gènes au cerveau de rat postnatal par des non-ventriculaire injection Plasmide et électroporation

Article DOI: 10.3791/2244
September 17th, 2010 •
1, 1,2, 1,2, 1
1Neuroscience Center, University of Helsinki, 2Faculty of Biological and Enviromental Sciences, University of Helsinki

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September 17th, 2010

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Ce protocole décrit une méthode non virale de la livraison des constructions génétiques à une certaine zone du cerveau de rongeur vivant. La méthode consiste à la préparation de plasmides, de fabrication micropipette, la chirurgie néonatale chez le rat nouveau-nés, la microinjection de la construction, et

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