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JoVE Journal Neuroscience
Gene Leverans till Postnatal råtthjärna av icke-ventrikulär Plasmid Injection och elektroporation
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Gene Leverans till Postnatal råtthjärna av icke-ventrikulär Plasmid Injection och elektroporation

Article DOI: 10.3791/2244
September 17th, 2010 •
1, 1,2, 1,2, 1
1Neuroscience Center, University of Helsinki, 2Faculty of Biological and Enviromental Sciences, University of Helsinki

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September 17th, 2010

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Detta protokoll beskriver en icke-virala metoden för leverans av genetiska konstruktioner till ett visst område av levande gnagare hjärnan. Metoden består av plasmid förberedelser, mikropipett tillverkning, neonatal hos råttungar kirurgi, mikroinjektion av konstruktionen, och

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