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从Midgastrula舞台果蝇胚胎神经文化的制备
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Biology
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JoVE Journal
Biology
Preparation of Neuronal Cultures from Midgastrula Stage Drosophila Embryos
Please note that all translations are automatically generated.
Click here for the English version.
从Midgastrula舞台果蝇胚胎神经文化的制备
DOI:
10.3791/226-v
•
13:07 min
•
July 04, 2007
•
Beatriz Sicaeros
,
Diane K. O’Dowd
1
Department of Development and Cell Biology, Department of Anatomy and Neurobiology
,
University of California, Irvine (UCI)
Chapters
00:00
Introduction
01:35
Embryo Dechorionation with Bleach
02:44
Preparation of Single Embryo Cultures
07:08
Obtaining Single Midgastrula Stage Embryo Cultures
08:48
Maintaining Healthy Embryonic Cultures
10:09
Viewing Embryonic Cultures
12:09
Conclusion
12:52
End Credits
Summary
Automatic Translation
English (Original)
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Automatic Translation
这个视频演示midgastrula阶段果蝇胚胎的主要神经文化的准备。生活文化的意见表明电镀和后2天在碳酸氢盐为基础的定义中等的增长有区别的神经元细胞1小时后。神经元电兴奋,并形成突触连接。
Tags
Neuronal Cultures
Midgastrula Stage
Drosophila Embryos
Primary Neuronal Cultures
Embryo Collection
Dechorionation
Cell Removal
Cell Dispersion
Medium Drop
Uncoated Glass Coverslip
Cell Density
Neuroblasts
Neuritic Processes
Neurite Outgrowth
Branching
Defined Medium
CO2 Incubator
Synaptic Connections
Voltage-gated Sodium Channels
Calcium Channels
Potassium Channels
Electrical Excitability
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