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优化转染的HEK - 293T细胞中的表达和重组的电压门控离子通道的电生理记录策略
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Biology
Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells
Please note that all translations are automatically generated.
Click here for the English version.
优化转染的HEK - 293T细胞中的表达和重组的电压门控离子通道的电生理记录策略
DOI:
10.3791/2314-v
•
12:59 min
•
January 19, 2011
•
Adriano Senatore
,
Adrienne N. Boone
,
J. David Spafford
1
Department of Biology
,
University of Waterloo
Chapters
00:00
Title
02:36
Transfection of HEK-293T with Recombinant Ion Channel cDNAs
04:52
Plating Transfected HEK-293T Cells in Preparation for Electrophysiological Recording
07:36
Electrophysiological Recording of Recombinant Channels in HEK-293T Cells
11:29
Representative Imaging and Patch Clamp Recordings
12:13
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
高效可靠的方法<em>在体外</em>表达和随后的重组中培养的人类胚胎肾细胞电压门控离子通道(HEK - 293T)的电生理记录。
Tags
Optimized
Transfection Strategy
Expression
Electrophysiological Recording
Recombinant Voltage-gated Ion Channels
HEK-293T Cells
In Vitro
Cultured Human Embryonic Kidney Cells
Glass Coverslips
Density
Electrophysiological Recording
Contact
Confounding Effects
Plasma Membrane
Whole-cell Patch Clamp Recording
Detectable Currents
Noise Levels
Incubation Periods
Membrane Expression
Cell-coverslip Adhesion
Membrane Stability
Temperature
Transfected Cells
High Confluency
Ion Channel Protein Expression
Glass Coverslips
Incubation Period
Cell Attachment
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