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January 19, 2011
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Método confiável para altamente eficiente<em> In vitro</em> Expressão e de gravação eletrofisiológicas subseqüente de recombinação dos canais de íons nas culturas de células embrionárias do rim (HEK-293T).
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OptimizedTransfection StrategyExpressionElectrophysiological RecordingRecombinant Voltage-gated Ion ChannelsHEK-293T CellsIn VitroCultured Human Embryonic Kidney CellsGlass CoverslipsDensityElectrophysiological RecordingContactConfounding EffectsPlasma MembraneWhole-cell Patch Clamp RecordingDetectable CurrentsNoise LevelsIncubation PeriodsMembrane ExpressionCell-coverslip AdhesionMembrane StabilityTemperatureTransfected CellsHigh ConfluencyIon Channel Protein ExpressionGlass CoverslipsIncubation PeriodCell Attachment
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Senatore, A., Boone, A. N., Spafford, J. D. Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells. J. Vis. Exp. (47), e2314, doi:10.3791/2314 (2011).
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