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Biology
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免疫印迹检测样品制备
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Western Blotting: Sample Preparation to Detection
Please note that all translations are automatically generated.
Click here for the English version.
免疫印迹检测样品制备
DOI:
10.3791/2359-v
•
07:45 min
•
October 14, 2010
•
Anna Eslami
,
Jesse Lujan
1
Research and Development
,
EMD Chemicals Inc.
Chapters
00:00
Title
00:58
Sample Preparation
01:52
SDS-PAGE
03:21
Protein Transfer
04:55
Membrane Blocking and Detection
06:56
Representative Western Blotting Results
07:26
Conclusion
Summary
Automatic Translation
English (Original)
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français (French)
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Automatic Translation
Western印迹分析技术,用于检测特定的蛋白质,在一个给定的样本,组织匀浆或提取。
Tags
Western Blotting
Sample Preparation
Detection
Proteins
Extract
Gel Electrophoresis
Native Proteins
Denatured Proteins
Polypeptide Length
3-D Structure
Membrane Transfer
Nitrocellulose
PVDF
Antibody Detection
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