/
/
تأسيس الخلايا الجذعية الجنينية الماوس العصبية الثقافة باستخدام الفحص Neurosphere
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:01Title
- 00:29Introduction
- 00:57Animal Dissection
- 02:40E14 Brain Dissection
- 04:42Tissue Dissociation
- 08:00Passaging Neurospheres
- 10:10Representative Results
- 11:31Conclusion
Tags
Embryonic Mouse Neural Stem Cell CultureNeurosphere AssayStem CellsUndifferentiated CellsCell GenesisAging ProcessInjury And DiseaseNeural Stem Cells (NSCs)Serum-free Culture SystemIsolation And Expansion Of NSCsEmbryonic CNSIn Vitro Expanded NSCsCNS Cell TypesReliable And Consistent ResultsE14 Mouse EmbryosBrain MicrodissectionGanglionic EminencesSingle Cell SuspensionNSA CulturePrimary Neurospheres
