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Chapters
- 00:05Title
- 02:01Embryo Collection & Lining Up
- 03:35Needle Preparation
- 04:40dsRNA Injection into Embryos and Phenotypic Analysis
- 06:36Representative RNAi Results
- 07:57Conclusion
RNA interference has been proven very effective to analyze gene function in Drosophila tracheal development. A detailed protocol used by Jiang lab to inject dsRNA into fly embryos to knockdown gene expression is illustrated. This technique has the potential for screening genes required for tissue and organ development in Drosophila.
