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JoVE Journal
Neuroscience
Author Produced

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Lokalisera Protein i 3D Neural Stem Cell Culture
 
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Lokalisera Protein i 3D Neural Stem Cell Culture: en hybrid Visualisering Methodology

Article DOI: 10.3791/2483
December 19th, 2010

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Summary December 19th, 2010

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Här beskriver vi hur man kan producera, expandera och immunolabel postnatal hippocampus neurala progenitorceller (NPC) i tre-dimensionell (3D) kultur. Nästa, med hjälp av hybrid-visualisering teknik, visar vi hur digitala bilder av immunolabelled kryosnitt kan användas för att rekonstruera och kartlägga de rumsliga position immunopositive celler i hela 3D neurosphere.

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