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Biology
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鼠标涎腺干细胞的分离
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Isolation of Mouse Salivary Gland Stem Cells
Please note that all translations are automatically generated.
Click here for the English version.
鼠标涎腺干细胞的分离
DOI:
10.3791/2484-v
•
09:52 min
•
February 08, 2011
•
Sarah Pringle
2
,
Lalitha S. Y. Nanduri
,
van der Zwaag Marianne
,
van Os Ronald
2
,
Rob P. Coppes
2
1
Department of Cell Biology
,
University Medical Center Groningen, University of Groningen
,
2
Department of Radiation Oncology
,
University Medical Center Groningen, University of Groningen
Chapters
00:05
Title
01:32
Mechanical and Enzymatic Salivary Gland Tissue Digestion
05:00
Preparation of Salivary Gland Tissue for Plating
07:32
Plating Salivary Gland Cells
08:39
Examination of Salispheres and Proliferating Cells Using Phase-Contrast Microscopy and Immunostaining
09:34
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
分离干细胞从小鼠唾液腺的优化协议描述。该方法采用酶法和机械消化,并允许包含干细胞的特征细胞salispheres的隔离。
Tags
Isolation
Mouse Salivary Gland Stem Cells
Salivary Glands
Cell Types
Production
Excretion
Saliva
Acinar Cells
Protein Production
Mucous Production
Ductal Cells
Epithelial-type Morphology
Saliva Composition
Diseases
Sjögren’s Syndrome
Radiotherapy Treatment
Xerostomia
Dry Mouth
Dental Caries
Regenerative Capacity
Stem Cell-like Populations
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