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Journal
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Biology
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影像生活细胞的形状变化
果蝇
胚胎
JoVE Journal
Biology
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JoVE Journal
Biology
Imaging Cell Shape Change in Living
Drosophila
Embryos
Please note that all translations are automatically generated.
Click here for the English version.
影像生活细胞的形状变化
果蝇
胚胎
DOI:
10.3791/2503-v
•
11:20 min
•
March 30, 2011
•
Lauren Figard
,
Anna Marie Sokac
2
1
Program in Cell & Molecular Biology
,
Baylor College of Medicine (BCM)
,
2
Verna & Marrs McLean Department of Biochemistry & Molecular Biology
,
Baylor College of Medicine (BCM)
Chapters
00:05
Title
00:45
Introduction
02:11
Making the Embryo Collection Cups
02:31
Adding GFP Flies to Embryo Collection Cups
04:43
Preparing a Mounting Chamber and Mounting Embryos
07:15
Imaging Embryos
07:55
Alternative Method: Mounting Embryos with Embryo -glue”
09:30
Results: Time-lapse Imaging of Cellularization
10:34
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
果蝇,果蝇,早期发展的特点是由许多细胞形状的变化,以及成像方法适合。本文将描述活果蝇胚胎的共焦成像所需的基本工具和方法,将集中在细胞形态变化称为cellularization。
Tags
Imaging Cell Shape Change
Living Drosophila Embryos
Confocal Imaging
Gene Expression
Signal Transduction
Cell Polarity
Cytoskeletal Remodeling
Membrane Trafficking
Cellularization
Plasma Membrane Growth
Syncytial Embryo
Cellular Blastoderm
Epithelial Cells
Myosin-2 Contractility
Exocytosis
Internal Stores
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