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改进的可视化和定量分析,药物使用Micropatterned细胞的影响
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal Bioengineering
Improved Visualization and Quantitative Analysis of Drug Effects Using Micropatterned Cells
DOI:

15:41 min

December 02, 2010

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Chapters

  • 00:05Title
  • 01:10Introduction
  • 03:52Cell Preparation and Seeding on Micropatterns
  • 06:14Blebbistatin Treatment, Cell fixation and Immunofluorescent Staining
  • 08:58Microscope Setup and Automated Image Acquisition
  • 11:02Automated Image Processing and Analysis
  • 13:40Representative Micropattern Imaging Results
  • 14:47Conclusion

Summary

Automatic Translation

可用于胶粘剂的微图案,细胞结构正常化,增加检测药物作用的敏感性,改善的重现性和简化自动化图像采集和分析。这种技术将有利于药物/ siRNA的筛选试验,进行常规的细胞培养支持,并因此遭受过度的细胞 - 细胞变性。

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