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高效率转导肝癌细胞的重组腺相关病毒血清型3载体
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal
Medicine
High-Efficiency Transduction of Liver Cancer Cells by Recombinant Adeno-Associated Virus Serotype 3 Vectors
Please note that all translations are automatically generated.
Click here for the English version.
高效率转导肝癌细胞的重组腺相关病毒血清型3载体
DOI:
10.3791/2538-v
•
19:02 min
•
March 22, 2011
•
Chen Ling
,
Yuan Lu
,
Binbin Cheng
,
Katherine E. McGoogan
,
Samantha W.Y. Gee
,
Wenqin Ma
,
Baozheng Li
,
George V. Aslanidi
,
Arun Srivastava
1
Department of Pediatrics, Division of Cellular and Molecular Therapy
,
University of Florida
Chapters
00:05
Title
01:33
Packaging of Recombinant Adeno-associated Virus R=Serotype 3 (rAAV3) Vectors
08:29
Determination of rAAV3 Vector Titers
15:11
rAAV3 Vector-mediated Transduction and Transgene Expression in Human Liver Cancer Cells
16:55
A Representative Quantitative DNA Slot-blot and Representative Transduction of Hepatoblastoma Cells with rAAV3
18:32
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这篇文章中,我们描述了鉴定的腺相关病毒血清型3(AAV3)针对人肝癌细胞的最有效的载体。
Tags
Recombinant Adeno-associated Virus Serotype 3 Vectors
Liver Cancer Cells
Gene Therapy
AAV2
AAV Serotypes
Human Liver Cancer
Hepatoblastoma
Hepatocellular Carcinoma
Cellular Co-receptor
High-efficiency Transduction
Reporter Gene
Potential Gene Therapy
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