/
/
Hög genomströmning MikroRNA Profilering: Optimerad Multiplex QRT-PCR vid metod i nanoliter Skala på Fluidigm Dynamic ArrayTM IFCS
This content is Free Access.
1The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research,University of California San Francisco , 2Center for Reproductive Sciences,University of California San Francisco , 3Department of Urology,University of California San Francisco , 4Department of Cell and Tissue Biology,University of California San Francisco , 5Fluidigm Corporation,Fluidigm Corporation , 6Department of Obstetrics and Gynecology,Hadassah-Hebrew University Medical Center, 7UCSF – Helen Diller Family Comprehensive Cancer Center,University of California San Francisco
Chapters
- 00:05Title
- 00:55RNA Concentration
- 02:03Gel Purification of pre-PCR Product
- 03:46Preparing the 96.96 Dynamic Array IFC, the Samples, and the 13x Assays
- 05:25qRT-PCR Analysis of miRNA Expression in Mouse ESC and Human Serum
- 06:10qRT-PCR Analysis
- 07:04Conclusion
Här beskriver vi ett optimerat multiplex omvänt transkriptas kvantitativ PCR (QRT-PCR) protokollet i kombination med en mikroflödessystem plattform som ett kostnadseffektivt och tidsbesparande high-throughput screening verktyg för mikro-RNA (miRNA) uttryck nivå, särskilt när man arbetar med begränsade mängder av prov.
Tags
High Throughput MicroRNA ProfilingMultiplex QRT-PCRFluidigm Dynamic ArrayTM IFCsMiRNAsQuantificationMicroRNA LevelsWild-typeSmall RNA Deficient Mouse Embryonic Stem Cells (mESC)AccuracyRobustnessOptimized MethodPurifying PrimersSingleplex Real-time DetectionMicrofluidic ChipNanoliter VolumesReagent CostsHigh Throughput MiRNA Expression Profiling
