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修正されたES / OP9共培養のプロトコルは、造血後代の強化された特性を提供します。
JoVE Journal
Biology
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JoVE Journal
Biology
Modified ES / OP9 Co-Culture Protocol Provides Enhanced Characterization of Hematopoietic Progeny
Please note that all translations are automatically generated.
Click here for the English version.
修正されたES / OP9共培養のプロトコルは、造血後代の強化された特性を提供します。
DOI:
10.3791/2559-v
•
08:36 min
•
June 07, 2011
•
Maureen R. Lynch
,
Judith C. Gasson
,
Helicia Paz
1
Department of Medicine, Hematology-Oncology
,
University of California, Los Angeles
,
2
Department of Biological Chemistry
,
University of California, Los Angeles
Chapters
00:05
Title
01:31
Preparing mStrawberry OP9 Cells
02:32
Setting up ES/mStrawberry OP9 Co-culture (Day 0)
03:55
Maintaining ES/mStrawberry OP9 Co-culture
06:16
Evaluating ES-derived Progeny from the Co-culture System
Summary
Automatic Translation
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Automatic Translation
mStrawberry OP9細胞は共培養からすべてのES由来の子孫の完全な評価が可能になります。
Tags
ES/OP9 Co-culture Protocol
Hematopoietic Progeny
In Vitro Differentiation
Early Hematopoietic Development
Flk-1+ Hemangioblasts
Terminally Differentiated Lineages
OP9 Stromal Cells
M-CSF
Embryonic Lethalities
Macrophage Overproduction
Replating Steps
Evaluation Of ES-derived Progeny
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