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April 16, 2011
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Summary
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وقد استخدمت لتحديد FRAP تنقل الإسفار البروتين الأخضر (GFP) الموسومة البروتينات في الخلايا المستزرعة. درسنا الكسور متنقلة / متحركة للGFP من خلال تحليل نسبة الانتعاش بعد photobleaching مضان. في هذه الدراسة ، تم تنفيذ FRAP في العمود الفقري من الخلايا العصبية قرن آمون.
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Cite this Article
Zheng, C., Petralia, R. S., Wang, Y., Kachar, B. Fluorescence Recovery After Photobleaching (FRAP) of Fluorescence Tagged Proteins in Dendritic Spines of Cultured Hippocampal Neurons. J. Vis. Exp. (50), e2568, doi:10.3791/2568 (2011).
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