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漂白后荧光标记蛋白的荧光恢复（FRAP）在培养海马神经元的树突棘
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Fluorescence Recovery After Photobleaching (FRAP) of Fluorescence Tagged Proteins in Dendritic Spines of Cultured Hippocampal Neurons
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漂白后荧光标记蛋白的荧光恢复（FRAP）在培养海马神经元的树突棘

DOI:
10.3791/2568-v

•

06:35 min

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April 16, 2011

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, , ,
1National Institute on Deafness and Other Communication Disorders,National Institutes of Health, Bethesda

Chapters

  • 00:05Title
  • 00:43Introduction
  • 01:15Neuron Transfection
  • 02:44FRAP Experiment on a Spine
  • 04:40Data Analysis
  • 05:28Representative Results: FRAP Measurements of EGFP Fluorescence in a Spine from a Cultured Hippocampal Neuron
  • 06:13Conclusion

Summary

Automatic Translation

Automatic Translation

FRAP已被用来量化绿色荧光蛋白（GFP）标记在培养细胞中的蛋白质的流动性。我们研究分析了GFP的荧光漂白后恢复的百分比移动/静止的分数。在这项研究中，FRAP刺海马神经元。

Tags

Fluorescence Recovery After Photobleaching (FRAP)GFP-tagged ProteinsDendritic SpinesCultured Hippocampal NeuronsMobilityExcitation LaserFluorescence Recovery RateProtein Turnover RateEGFP VectorZeiss 710 Confocal MicroscopePhotobleachingTime Lapse ImagesMobile FractionImmobile FractionImageJ SoftwareGraphpad Software

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