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JoVE Journal
Neuroscience

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漂白后荧光标记蛋白的荧光恢复(FRAP)在培养海马神经元的树突棘
 
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漂白后荧光标记蛋白的荧光恢复(FRAP)在培养海马神经元的树突棘

Article DOI: 10.3791/2568-v 06:36 min April 16th, 2011
April 16th, 2011

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Summary

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Click here for the English version.

FRAP已被用来量化绿色荧光蛋白(GFP)标记在培养细胞中的蛋白质的流动性。我们研究分析了GFP的荧光漂白后恢复的百分比移动/静止的分数。在这项研究中,FRAP刺海马神经元。

Tags

神经科学杂志,50期,脊柱,FRAP,海马神经细胞,活细胞成像,蛋白质的流动性
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