54,139 Views
•
06:35 min
•
April 16, 2011
DOI:
Summary
Automatically generated
FRAP была использована для количественного мобильность Зеленый флуоресцентный белка (GFP), помеченные белков в культуре клеток. Мы рассмотрели мобильных / неподвижные доли GFP путем анализа флуоресценции процент выздоровления после фотообесцвечивания. В этом исследовании, FRAP была выполнена на шипы нейронов гиппокампа.
Explore More Videos
Related Videos
11:48
Analysis of Dendritic Spine Morphology in Cultured CNS Neurons
Related Videos
34861 Views
09:52
Inducing Dendritic Growth in Cultured Sympathetic Neurons
Related Videos
12665 Views
11:58
Lateral Diffusion and Exocytosis of Membrane Proteins in Cultured Neurons Assessed using Fluorescence Recovery and Fluorescence-loss Photobleaching
Related Videos
82898 Views
12:51
Voltage-sensitive Dye Recording from Axons, Dendrites and Dendritic Spines of Individual Neurons in Brain Slices
Related Videos
16627 Views
12:58
Imaging pHluorin-tagged Receptor Insertion to the Plasma Membrane in Primary Cultured Mouse Neurons
Related Videos
13098 Views
16:33
Detection of Protein Palmitoylation in Cultured Hippocampal Neurons by Immunoprecipitation and Acyl-Biotin Exchange (ABE)
Related Videos
35214 Views
11:56
Differential Labeling of Cell-surface and Internalized Proteins after Antibody Feeding of Live Cultured Neurons
Related Videos
15307 Views
14:11
Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy
Related Videos
15712 Views
13:24
Fluorescence and Bioluminescence Imaging of Subcellular Ca2+ in Aged Hippocampal Neurons
Related Videos
8258 Views
07:30
Measuring Synaptic Vesicle Endocytosis in Cultured Hippocampal Neurons
Related Videos
9756 Views
Read Article
Cite this Article
Zheng, C., Petralia, R. S., Wang, Y., Kachar, B. Fluorescence Recovery After Photobleaching (FRAP) of Fluorescence Tagged Proteins in Dendritic Spines of Cultured Hippocampal Neurons. J. Vis. Exp. (50), e2568, doi:10.3791/2568 (2011).
Copy