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Chapters
- 00:00Title
- 00:13Introduction
- 00:41Tail Vein Injection for Adoptive Transfer of CFSE-labelled T Lympho
- 03:45Removal of Lymph Nodes
- 06:42Removing the Inguinal Lymph Nodes
- 08:48Removing the Axillary Lymph Nodes
- 11:24Removing the Cervical Lymph Nodes
- 13:07Preparation of Lymph Nodes for In Situ Imaging
- 15:362-photon Imaging of Lymph Nodes in Situ
Två-foton avbildning har avslöjat lymfocyter motilitet och cellulära interaktioner inom den lymfkörtel i basala förhållanden och under ett immunsvar 1. Här visar vi adoptiv transfer av T-celler, isolering av lymfkörtlar, och avbildning motilitet av CD4 + T celler i explanterade lymfkörtel.
Tags
Dissection2-Photon ImagingPeripheral Lymph NodesMiceAdoptive TransferLabeled T CellsIsolationImaging MotilityCD4+ T CellsExplanted Lymph NodeTwo-photon Imaging Of Immune CellsFluorescently Labeled CellsCell Tracker DyeFluorescent ProteinTail Vein InjectionLymphoid OrgansIsolation Of Lymph NodeExcised Lymph NodeOxygenation Of Perfused MediaTemperatureLaser Power3D Video ImagesNaive CD4+ T Cells
