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Biology
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夹层和小鼠的外周淋巴结的双光子成像
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Dissection and 2-Photon Imaging of Peripheral Lymph Nodes in Mice
Please note that all translations are automatically generated.
Click here for the English version.
夹层和小鼠的外周淋巴结的双光子成像
DOI:
10.3791/265-v
•
16:48 min
•
August 23, 2007
•
Melanie P. Matheu
,
Ian Parker
,
Michael D. Cahalan
1
Department of Physiology and Biophysics
,
University of California, Irvine (UCI)
,
2
Department of Neurobiology and Behaviour
,
University of California, Irvine (UCI)
Chapters
00:00
Title
00:13
Introduction
00:41
Tail Vein Injection for Adoptive Transfer of CFSE-labelled T Lympho
03:45
Removal of Lymph Nodes
06:42
Removing the Inguinal Lymph Nodes
08:48
Removing the Axillary Lymph Nodes
11:24
Removing the Cervical Lymph Nodes
13:07
Preparation of Lymph Nodes for In Situ Imaging
15:36
2-photon Imaging of Lymph Nodes in Situ
Summary
Automatic Translation
English (Original)
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Automatic Translation
双光子成像内已发现的基础条件下的淋巴结和淋巴细胞的活力和细胞相互作用过程中的
免疫
反应1。在这里,我们证明收养的T细胞,淋巴结的隔离和成像蠕动的CD4 + T细胞在explanted淋巴结转移。
Tags
Dissection
2-Photon Imaging
Peripheral Lymph Nodes
Mice
Adoptive Transfer
Labeled T Cells
Isolation
Imaging Motility
CD4+ T Cells
Explanted Lymph Node
Two-photon Imaging Of Immune Cells
Fluorescently Labeled Cells
Cell Tracker Dye
Fluorescent Protein
Tail Vein Injection
Lymphoid Organs
Isolation Of Lymph Node
Excised Lymph Node
Oxygenation Of Perfused Media
Temperature
Laser Power
3D Video Images
Naive CD4+ T Cells
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