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在斑马鱼胚胎的代谢标记和Bioorthogonal点击化学成像聚糖
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Imaging Glycans in Zebrafish Embryos by Metabolic Labeling and Bioorthogonal Click Chemistry
Please note that all translations are automatically generated.
Click here for the English version.
在斑马鱼胚胎的代谢标记和Bioorthogonal点击化学成像聚糖
DOI:
10.3791/2686-v
•
06:46 min
•
June 06, 2011
•
Hao Jiang
,
Lei Feng
,
David Soriano del Amo
,
Ronald D. Seidel III
,
Florence Marlow
,
Peng Wu
1
Department of Biochemistry
,
Albert Einstein College of Medicine, Yeshiva University
,
2
Macromolecular Therapeutics Development Facility
,
Albert Einstein College of Medicine, Yeshiva University
,
3
Developmental and Molecular Biology
,
Albert Einstein College of Medicine, Yeshiva University
Chapters
00:05
Title
01:58
Egg Collection and Dechorionation
02:34
Microinjection with GDP-FucAI
03:30
BTTES-Cu(I)-catalyzed Click Chemistry Reaction
04:34
Imaging
05:06
In Vivo Imaging of Fucosylated Glycans During Zebrafish Embryogenesis
06:11
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
点击化学的方法,允许炔标签聚糖在斑马鱼胚胎的快速,非侵入性和强大的标签描述。在这项研究中,已故的原肠胚形成阶段,在斑马鱼胚胎的包围层Fucosylated聚糖成像。
Tags
Glycans
Zebrafish Embryos
Metabolic Labeling
Bioorthogonal Click Chemistry
Chemical Reporter Strategy
Azide-tagged Monosaccharides
Alkyne-tagged Monosaccharides
Glycan Biosynthetic Machinery
Cell Surface Glycoconjugates
Fluorescent Probes
Affinity Probes
Noninvasive Imaging
Fucosylated Glycans
Microinjection
GDP-5-alkynylfucose (GDP-FucAl)
Azide-conjugated Fluorophores
Cu(I)-catalyzed Azide-alkyne Cycloaddition (CuAAC)
Confocal Microscopy
Sialic Acid-containing Glycans
N-acetylgalactosamine-containing Glycans
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