A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:59Injection of mRNA Encoding the Fluorescently-tagged Actin Binding Protein
- 03:47Induction of Cell Extrusion in Zebrafish Larvae Using G418
- 04:47Mounting Zebrafish Larvae for Imaging
- 05:34Live Imaging of Actin Dynamics and Individual Epithelial Cell Behaviors during Cell Extrusion Using a Spinning Disc Confocal Microscope
- 08:22Representative Epidermal Images
- 08:55Conclusion
Cellule morenti vengono estrusi da tessuti epiteliali dalla contrazione concertata delle cellule vicine senza interrompere la funzione di barriera. L'ottica degli zebrafish di sviluppo, prevede un ottimo sistema per visualizzare estrusione nel vivere epiteli. Qui si descrivono i metodi per indurre ed estrusione immagine nell'epidermide zebrafish larvale a risoluzione cellulare.
Tags
Live ImagingCell ExtrusionEpidermisDeveloping ZebrafishDamaged CellsBarrier FunctionDying CellsActin And MyosinEpithelial SheetCell Extrusion ProcessOptical ClarityLarval Zebrafish EpidermisFluorescent Protein Labeled ProbeF-actinTransgenic Zebrafish EmbryosGreen Fluorescent ProteinApoptosisG418Time-lapse ImagingSpinning Disc Confocal MicroscopeActin DynamicsEpithelial Cell BehaviorsApoptotic Cell Elimination
