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A Real-time elektriske impedans Baseret Teknik til måling Invasionen af ​​Endotel cellemonolag ved kræftceller
JoVE Journal
Medicine
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JoVE Journal Medicine
A Real-time Electrical Impedance Based Technique to Measure Invasion of Endothelial Cell Monolayer by Cancer Cells

A Real-time elektriske impedans Baseret Teknik til måling Invasionen af ​​Endotel cellemonolag ved kræftceller

DOI:
10.3791/2792-v

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08:51 min

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April 01, 2011

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,
1Lombardi Comprehensive Cancer Center,Georgetown University

Chapters

  • 00:05Title
  • 01:46Preparation
  • 03:56Generating HUVEC Monolayer
  • 05:52Addition of Invading Cells and Data Normalization
  • 07:27Representative Invasion Assay Results
  • 08:18Conclusion

Summary

Automatic Translation

Automatic Translation

Denne artikel beskriver en<em> In vitro</em> Teknik til overvågning kræftceller invaderer gennem et monolag af endotelceller. Dataene opnået i realtid som en funktion af ændringer i impedans på overfladen af ​​elektroderne ved brønden bund.

Tags

Real-timeElectrical ImpedanceTechniqueMeasureInvasionEndothelial Cell MonolayerCancer CellsMetastatic DisseminationBasement Membrane DegradationTumor Cell AttachmentVascular EndotheliumEndothelial Junction RetractionInvasion And MigrationCirculatory SystemAdherence To Capillary WallsMetastatic Tumors FormationTumor Cell-endothelial Cell InteractionIn Vitro ReplicationMonolayer Of Human Umbilical Vein Endothelial Cells (HUVEC)Cancer Cell ChallengeElectron MicroscopyPhase-contrast MicroscopyIn Vivo Metastatic Process RepresentationElectrical Impedance Fluctuations MeasurementXCELLigence InstrumentRoche Manufacturing

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