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Neuroscience
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培養中枢神経系ニューロンの樹状突起スパイン形態の解析
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Neuroscience
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JoVE Journal
Neuroscience
Analysis of Dendritic Spine Morphology in Cultured CNS Neurons
Please note that all translations are automatically generated.
Click here for the English version.
培養中枢神経系ニューロンの樹状突起スパイン形態の解析
DOI:
10.3791/2794-v
•
11:48 min
•
July 13, 2011
•
Deepak P. Srivastava
,
Kevin M. Woolfrey
,
Peter Penzes
2
1
Department of Physiology
,
Northwestern University Feinberg School of Medicine
,
2
Department of Psychiatry and Behavioral Sciences
,
Northwestern University Feinberg School of Medicine
Chapters
00:05
Title
02:00
Transfection of Primary Cultured Cortical Neurons
03:14
Treatment of Primary Cultured Cortical Neurons Grown on 18 mm Coverslips
03:46
Fixation and Immunocytohistochemistry
05:21
Quantitative Analysis of Spine Morphologies
06:58
Time-lapse Imaging
09:43
Representative Results: Analysis of Dendritic Spine Morphology
11:04
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
数々の最近の研究では、脳の病理学に関連したシナプス蛋白の変異を同定した。初代培養皮質ニューロンは樹状突起スパイン形態と運動性にこれらの疾患関連タンパク質の影響を調べることで最大限の柔軟性を提供します。
Tags
Dendritic Spines
Morphology
Cultured Neurons
Excitatory Connections
Synapses
Actin
Hebbian Plasticity
Neuromodulatory Signals
Neural Circuits
Information Processing
Synaptic Proteins
Disease States
Schizophrenia
Autism Spectrum Disorders
Aberrant Spine Plasticity
Pathophysiology
Cultured Cortical Neurons
High-resolution Imaging
Fixed Cells
Time-lapse Imaging
Article
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