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Neuroscience
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在培养的中枢神经系统的神经元树突棘形态分析
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Analysis of Dendritic Spine Morphology in Cultured CNS Neurons
Please note that all translations are automatically generated.
Click here for the English version.
在培养的中枢神经系统的神经元树突棘形态分析
DOI:
10.3791/2794-v
•
11:48 min
•
July 13, 2011
•
Deepak P. Srivastava
,
Kevin M. Woolfrey
,
Peter Penzes
2
1
Department of Physiology
,
Northwestern University Feinberg School of Medicine
,
2
Department of Psychiatry and Behavioral Sciences
,
Northwestern University Feinberg School of Medicine
Chapters
00:05
Title
02:00
Transfection of Primary Cultured Cortical Neurons
03:14
Treatment of Primary Cultured Cortical Neurons Grown on 18 mm Coverslips
03:46
Fixation and Immunocytohistochemistry
05:21
Quantitative Analysis of Spine Morphologies
06:58
Time-lapse Imaging
09:43
Representative Results: Analysis of Dendritic Spine Morphology
11:04
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
许多最近的研究已经确定了在与大脑病症相关突触蛋白的基因突变。原代培养皮层神经元在研究这些疾病相关的蛋白质对树突棘的形态和运动的影响提供了极大的灵活性。
Tags
Dendritic Spines
Morphology
Cultured Neurons
Excitatory Connections
Synapses
Actin
Hebbian Plasticity
Neuromodulatory Signals
Neural Circuits
Information Processing
Synaptic Proteins
Disease States
Schizophrenia
Autism Spectrum Disorders
Aberrant Spine Plasticity
Pathophysiology
Cultured Cortical Neurons
High-resolution Imaging
Fixed Cells
Time-lapse Imaging
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