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增强生物安全与聚合物复合物Ecotropic慢病毒的人体细胞的转导
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Transduction of Human Cells with Polymer-complexed Ecotropic Lentivirus for Enhanced Biosafety
DOI:

14:03 min

July 24, 2011

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Chapters

  • 00:05Title
  • 01:18Production of Lentivirus
  • 04:03Titering the Lentivirus
  • 04:57Transduction of Human Target Cells with Murine Retrovirus Receptor Slc7a1
  • 05:50Polymer Complex Titration to Determine Toxicity
  • 10:15Ecotropic Transduction with Polymer Complexation
  • 11:08Results of the Procedure
  • 13:32Conclusion

Summary

Automatic Translation

慢病毒是一个有价值的研究工具,为探索基因功能,然而,研究人员可能希望避免生产pantropic慢病毒,已知的编码或怀疑致癌基因。作为替代方案,我们目前使用ecotropic慢病毒修改的人体细胞表达ecotropic的受体mSlc7a1一个更安全的协议。

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