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September 10, 2011
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Summary
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Environmental Bakterioplankton sind mit einem Modell des gelösten organischen Kohlenstoffs (DOC)-Verbindung und einer DNA Markierungsreagens, Bromdesoxyuridin (BrdU) inkubiert. Anschließend werden DOC-abbauenden Zellen aus dem Bulk-Community auf ihre erhöhte BrdU-Einbaus mittels Fluoreszenz-activated cell sorting (FACS) basiert getrennt. Diese Zellen werden dann durch nachfolgende molekulare Analysen identifiziert.
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Cite this Article
Robbins, S., Jacob, J., Lu, X., Moran, M. A., Mou, X. Bromodeoxyuridine (BrdU) Labeling and Subsequent Fluorescence Activated Cell Sorting for Culture-independent Identification of Dissolved Organic Carbon-degrading Bacterioplankton. J. Vis. Exp. (55), e2855, doi:10.3791/2855 (2011).
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