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Chapters
- 00:05Title
- 01:33Growing Cotton Seedlings
- 02:43Constructing the VIGS Vector Carrying the GrCla1 Gene
- 04:55Virus Induced Gene Silencing Inoculation
- 08:40Examples of Albino Phenotypes on VIGS Silenced Cotton Plants
- 09:26Conclusion
Vi presenterar detaljerade protokoll för Agrobacterium-medierad virusinducerade geners uttryck (VIGS) analys i bomull. Tobaken skallra virus (TRV)-härledda VIGS vektorer utplacerades att inducera RNA tysta av bomull GrCLA1, Cloroplastos alterados 1-genen. Den albino fenotyp orsakas av tysta GrCLA1 observerades vid plantan skede inom 2 veckor efter ympningen.
Tags
Agrobacterium-mediatedVirus-Induced Gene Silencing AssayCottonGossypium HirsutumMolecular BreedingGene Functional AnalysisGenome SizeGene DuplicationPolyploidyGrowth CycleGenetic TransformationHigh ThroughputFunctional Genetic/genomic StudyVIGSPost-Transcriptional Gene Silencing (PTGS)Viral ProliferationAgrobacterium-mediated VIGSDicots SpeciesSolanaceaeArabidopsisLegume SpeciesMonocots SpeciesBarleyWheatMaizeFunctional Genomic StudiesTobacco Rattle Virus (TRV)
