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Agrobacterium-medierad virusinducerade geners uttryck test på Bomull
JoVE Journal
Biology
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JoVE Journal Biology
Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton
DOI:

10:18 min

August 20, 2011

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Chapters

  • 00:05Title
  • 01:33Growing Cotton Seedlings
  • 02:43Constructing the VIGS Vector Carrying the GrCla1 Gene
  • 04:55Virus Induced Gene Silencing Inoculation
  • 08:40Examples of Albino Phenotypes on VIGS Silenced Cotton Plants
  • 09:26Conclusion

Summary

Automatic Translation

Vi presenterar detaljerade protokoll för Agrobacterium-medierad virusinducerade geners uttryck (VIGS) analys i bomull. Tobaken skallra virus (TRV)-härledda VIGS vektorer utplacerades att inducera RNA tysta av bomull GrCLA1, Cloroplastos alterados 1-genen. Den albino fenotyp orsakas av tysta GrCLA1 ​​observerades vid plantan skede inom 2 veckor efter ympningen.

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