JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
JA - 日本語
TR - Türkçe
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
JA - 日本語
TR - Türkçe
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
JA - 日本語
TR - Türkçe
Journal
/
Biology
/
Immunohistochemistry : 벡터 연구소에서 Vectastain ABC 키트를 사용하여 파라핀 섹션
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Immunohistochemistry: Paraffin Sections Using the Vectastain ABC Kit from Vector Labs
Please note that all translations are automatically generated.
Click here for the English version.
Immunohistochemistry : 벡터 연구소에서 Vectastain ABC 키트를 사용하여 파라핀 섹션
DOI:
10.3791/308-v
•
21:38 min
•
October 01, 2007
•
Victor Chi
,
K. George Chandy
1
Department of Physiology and Biophysics
,
University of California, Irvine (UCI)
Chapters
00:02
Title
00:06
Introduction
00:49
Dewaxing and Rehydrating Paraffin Sections
03:20
Unmasking Antigen with Heat-Induced Antigen Retrieval
05:14
Blocking Endogenous Peroxidase Activity and Nonspecific Binding of
08:17
Adding Primary and Secondary Antibodies
11:39
Preparing and Incubating Slides in the ABC Reagent
13:21
Preparing and Reacting Slides with DAB Substrate Reagent
16:10
Counterstaining and Mounting the Slides
20:46
Visualizing Anti-Tyrosine Hydroxylase Slides
21:07
Conclusion
Tags
Immunohistochemistry
IHC
Paraffin Sections
Vectastain ABC Kit
Vector Labs
Protein Expression
Specific Antibodies
Direct Method
Indirect Method
Primary Antibodies
Biotin-conjugated Secondary Antibodies
Frozen Sections
Paraffin-embedded Sections
Glass Slides
Dewaxing
Hydration
Alcohol Gradient
Antigen Retrieval
Endogenous Peroxidase Activity
Non-specific Binding Sites
T Cell Marker CD8
Tyrosine Hydroxylase
Secondary Antibodies Conjugated To Biotin
Avidin-conjugated Horseradish Peroxidase (HRP)
Diaminiobenzidine (DAB)
Contrast
Coverslip
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
Qiagen Miniprep 키트를 사용하여 세균의 플라스미드 식민지에서 DNA를 정화
StemCell 기술에서 RosetteSep 키트와 함께 인간의 피를에서 NK 세포의 농축
바이오 - 래드 총 RNA 키트를 사용 Neuroprecursor 셀에서 RNA 추출
형광 항체를 사용하여 마우스 표피의 파라핀 섹션에 Immunohistochemistry
의 파라핀 - 임베디드 및 냉동 섹션
Drosophila
성인 근육
유전과 Epigenetic 분석을위한 파라핀 임베디드 재료에서 DNA 추출
전 · Cryo 키트를 사용하여 마우스 정자 Cryopreservation 및 복구
STEMCCA Lentiviral 벡터를 사용하여 말초 혈에서 인간 유도 만능 줄기 세포의 생성
민감한 면역를 사용하여 시토신의 수정 된 형태의 검출
마이 오신 중쇄 면역를 사용하여 쥐의 근육 크로스 섹션에서 근육 섬유 인구 분석을위한 신속한 자동화 프로토콜
Read Article