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蛋白质和细胞坐月子创建二维图案衬底
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Creating Two-Dimensional Patterned Substrates for Protein and Cell Confinement
DOI:

08:36 min

September 06, 2011

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Chapters

  • 00:05Title
  • 01:08Preparation of the Patterned Master
  • 02:30Preparation of PDMS Stamp
  • 03:11Preparation of the Patterned Gold Substrate
  • 05:08Applying Protein and Cells to the Patterned Substrate
  • 06:12Results of Creating Two-Dimentional Patterned Substrates
  • 08:06Conclusion

Summary

Automatic Translation

金长链烷烃硫醇形成的自组装单层膜(SAMS)提供了定义良好的蛋白质模式的形成和细胞禁闭基板。使用一个邮票回填与聚二甲基硅氧烷(PDMS)微接触印刷hexadecanethiol乙二醇终止烷烃硫醇单体生产蛋白质和细胞吸附加盖hexadecanethiol地区唯一的模式。

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