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监测RNA结构平衡的变动情况“过氧化”和“氧化”羟基自由基足
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Biology
Monitoring Equilibrium Changes in RNA Structure by ‘Peroxidative’ and ‘Oxidative’ Hydroxyl Radical Footprinting
Please note that all translations are automatically generated.
Click here for the English version.
监测RNA结构平衡的变动情况“过氧化”和“氧化”羟基自由基足
DOI:
10.3791/3244-v
•
13:41 min
•
October 17, 2011
•
Ravichandra Bachu*
1
,
Frances-Camille S. Padlan*
2
,
Sara Rouhanifard
,
Michael Brenowitz
,
Jörg C. Schlatterer
1
Department of Chemistry
,
Hunter College
,
2
Department of Biochemistry
,
Albert Einstein College of Medicine
Chapters
00:05
Title
02:24
RNA Preparation for Footprinting Experiment
04:24
The Footprinting Experiment
08:09
Data Analysis
10:38
Analysis of RNA Fragments after Peroxidative Fenton Footprinting Reaction
12:23
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本协议描述了如何量化的镁(二)依赖RNA的三级结构的形成由两个羟基自由基足迹方法。
Tags
Monitoring
Equilibrium Changes
RNA Structure
Peroxidative
Oxidative
Hydroxyl Radical Footprinting
RNA Molecules
Genetic Information
Tertiary Structures
Biologic Role
Regulator
Binder
Catalyst
Nucleic Acids
Phosphodiester Backbone
Single Nucleotide Resolution
Intermolecular Contact Surface
Equilibrium Transitions
Kinetic Transitions
Solution Variable
Time
Probe Exposure
Polymer Sampling
Video Article
Sample Preparation
Mg(II)-mediated Folding Isotherms
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