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监测RNA结构平衡的变动情况“过氧化”和“氧化”羟基自由基足
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JoVE Journal Biology
Monitoring Equilibrium Changes in RNA Structure by ‘Peroxidative’ and ‘Oxidative’ Hydroxyl Radical Footprinting
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监测RNA结构平衡的变动情况“过氧化”和“氧化”羟基自由基足

DOI:
10.3791/3244-v

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13:41 min

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October 17, 2011

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, , , ,
1Department of Chemistry,Hunter College , 2Department of Biochemistry,Albert Einstein College of Medicine

Chapters

  • 00:05Title
  • 02:24RNA Preparation for Footprinting Experiment
  • 04:24The Footprinting Experiment
  • 08:09Data Analysis
  • 10:38Analysis of RNA Fragments after Peroxidative Fenton Footprinting Reaction
  • 12:23Conclusion

Summary

Automatic Translation

Automatic Translation

本协议描述了如何量化的镁（二）依赖RNA的三级结构的形成由两个羟基自由基足迹方法。

Tags

MonitoringEquilibrium ChangesRNA StructurePeroxidativeOxidativeHydroxyl Radical FootprintingRNA MoleculesGenetic InformationTertiary StructuresBiologic RoleRegulatorBinderCatalystNucleic AcidsPhosphodiester BackboneSingle Nucleotide ResolutionIntermolecular Contact SurfaceEquilibrium TransitionsKinetic TransitionsSolution VariableTimeProbe ExposurePolymer SamplingVideo ArticleSample PreparationMg(II)-mediated Folding Isotherms

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