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Monitoring Equilibrium veranderingen in RNA structuur door 'Peroxidative' en 'Oxidatieve' hydroxyl radicaal Footprinting
JoVE Journal
Biology
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JoVE Journal Biology
Monitoring Equilibrium Changes in RNA Structure by ‘Peroxidative’ and ‘Oxidative’ Hydroxyl Radical Footprinting
DOI:

13:41 min

October 17, 2011

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Chapters

  • 00:05Title
  • 02:24RNA Preparation for Footprinting Experiment
  • 04:24The Footprinting Experiment
  • 08:09Data Analysis
  • 10:38Analysis of RNA Fragments after Peroxidative Fenton Footprinting Reaction
  • 12:23Conclusion

Summary

Automatic Translation

Dit protocol beschrijft hoe het kwantificeren van de Mg (II)-afhankelijke vorming van RNA tertiaire structuur door twee methoden van hydroxyl-radicaal voetafdruk.

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