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在小鼠心脏的动作电位和钙瞬变的光学测绘
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Optical Mapping of Action Potentials and Calcium Transients in the Mouse Heart
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在小鼠心脏的动作电位和钙瞬变的光学测绘

DOI:
10.3791/3275-v

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08:13 min

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September 13, 2011

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, , ,
1Department of Biomedical Engineering,Washington University in St. Louis

Chapters

  • 00:05Title
  • 01:24Preparing the Perfusion and Imaging Setup
  • 03:23Langendorff Perfusion of the Isolated Heart
  • 05:25Loading Voltage and Calcium Sensitive Dyes
  • 06:38Optical Imaging and Data Analysis
  • 07:47Conclusion

Summary

Automatic Translation

Automatic Translation

本文详细解剖程序，工具设置，并在光学测绘跨膜电位（VM）和细胞内钙瞬变（CAT），在隔离的Langendorff完整的实验条件下灌注小鼠心脏。

Tags

Optical MappingAction PotentialsCalcium TransientsMouse HeartCardiovascular StudiesFluorescence ImagingGenetic EngineeringPhysiological PhenotypingTransmembrane Potential (Vm)Excitation-contraction CouplingLangendorff Perfused Mouse HeartsHeart FailureArrhythmiasMetabolic DiseaseHeart DiseasesActivation VisualizationConduction VelocityAction Potential DurationInstrumentation SetupExperimental ConditionsSpatio-temporal ResolutionCMOS Imaging Technology

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