JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
miRNA的目标使用的任务目标ID库的全基因组屏幕
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
Please note that all translations are automatically generated.
Click here for the English version.
miRNA的目标使用的任务目标ID库的全基因组屏幕
DOI:
10.3791/3303-v
•
08:40 min
•
April 06, 2012
•
Matthew J. Coussens*
1
,
Kevin Forbes*
1
,
Carol Kreader*
1
,
Jack Sago
,
Carrie Cupp
,
John Swarthout
1
Sigma-Aldrich
Chapters
00:05
Title
01:39
Transfection with Target ID Library and Selection for Stable Cell Lines
03:59
Selection Reagent Kill Curves
04:52
Transfection of Library Cells with miRNA Expression Constructs and Selection for Stable Cell Lines and miRNA Targets
06:53
Library Screen for miR-373 Targets
08:19
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
目标ID图书馆是一个基于质粒,用于识别miRNA靶基因的克隆基因的全基因组的集合。在这里,我们展示它的使用和应用。
Tags
Genome-wide Screen
MiRNA Targets
MISSION Target ID Library
Plasmid-based Library
CDNA Library
Thymidine Kinase-zeocin
Stable Transformants
MiRNA Of Interest
CDNAs Containing MiRNA’s Target
Sequencing
Human Transcriptome
Oligo-dT Priming
Total RNA
Sigma-Aldrich Webpage
Illumina Sequencing
Unique Genes
UCSC RefGene
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
(充电)酰化tRNA的使用微阵列技术水平的全基因组分析
采用芯片识别亚型特定的靶基因的全基因组分析
特派团LentiPlex汇集在哺乳动物细胞中的shRNA文库筛选
定量和自动化高通量的全基因组RNAi屏幕
C。线虫</em
一种新型的全基因组分析的贝叶斯变点算法的多元ChIPseq数据类型
全基因组基因缺失<em>链球菌血统</em通过高通量PCR
检测miRNA靶在高通量的使用3'LIFE分析
药物与DNA相互作用的细胞全基因组映射与COSMIC(小分子交联,染色质隔离)
从真核细胞染色体外的环状DNA基因组范围内的净化
葡萄全基因组鉴定与表达的综合工作流 E3 泛素连接基因家族的 Meta 分析
Read Article