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Imaging Pheromone Sensing i en mus Vomeronasal Akut Tissue Slice Forberedelse
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Chapters
- 00:05Title
- 02:01Dissection of the Mouse Vomeronasal Organ (VNO)
- 03:37VNO Tissue Slice Preparation
- 04:37Immunohistochemistry of VNO Floating Slices
- 05:19Calcium Imaging of VNO Slices
- 06:23Representative Immunostaining and Calcium Imaging of VNO Slice
- 09:00Conclusion
Hos mus, er evnen til at detektere feromoner hovedsageligt medieres af vomeronasal organ (VNO). Her er en akut væv skive forberedelse af VNO for udførelsen af calcium imaging beskrevet. Denne fysiologiske tilgang giver mulighed for observationer af delpopulationer og / eller individuelle neuroner i et levende væv og er praktisk for receptor-ligand identifikation.
Tags
Pheromone SensingMousePheromonesIntra-species CommunicationBiological FluidsUrinePheromonal CommunicationKin InteractionsHierarchical OrganizationsSexual InteractionsSurvival Of SpeciesVomeronasal Organ (VNO)Nasal CavityCartilaginous CapsuleSensory NeuroepitheliumVomeronasal Bipolar Sensory Neurons (VSNs)DendriteMicrovilliChemoreceptorLigandsVomeronasal Receptor FamiliesV1RsV2Rs
