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躯干神经嵴细胞迁移使用一个修改过的Zigmond室法分析
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Analysis of Trunk Neural Crest Cell Migration using a Modified Zigmond Chamber Assay
DOI:

12:17 min

January 19, 2012

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Chapters

  • 00:05Title
  • 01:55Isolation of Trunk Neural Tubes for Overnight Culture on Coverslips
  • 06:05Loading the Modified Zigmond Chamber and Time-lapse Analysis of Cell Migration
  • 09:50Representative Results
  • 11:50Conclusion

Summary

Automatic Translation

取出的细胞迁移(躯干神经嵴细胞)的方法来分析。这种方法是廉价的,温柔的,和能够区分趋化学增活现象和其他因素的影响,如在主躯干神经嵴细胞培养的细胞 - 细胞相互作用所产生的迁徙极性的。

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