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December 07, 2011
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Models of tumor cell invasion into three-dimensional extracellular matrix better reflect the in vivo situation than two-dimensional motility assays. Using matrix invasion assays combined with confocal imaging of fluorescently-labeled cells, detailed information on invasion modes and the distinct contributions of leading versus following cells can be obtained.
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ModelingImaging3-dimensionalCollective Cell InvasionCancer MalignancyInvasionMetastasisGliomaBreast CancerLung CancerMetastasis ProcessTumor CellsOrgan FailureMorbidityMortalityMolecular ProcessesProtein MediatorsDiagnosisTreatmentInvasion AssaysIn Vivo SituationTwo-dimensional Cell Motility AssaysExtracellular Matrix (ECM) Protein RemodelingTumor Cell InvasionIndividual Cells MovementCollective Invasion
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Scott, R. W., Crighton, D., Olson, M. F. Modeling and Imaging 3-Dimensional Collective Cell Invasion. J. Vis. Exp. (58), e3525, doi:10.3791/3525 (2011).
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