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Metoder til Undersøgelse af Neuronal morfogenese:<em> Ex vivo</em> RNAi Elektroporation i embryoniske murin Cerebral Cortex
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Chapters
- 00:05Title
- 01:53Dissection and Electroporation
- 04:10Embedding and Sectioning Electroporated Cortices
- 06:53Culturing and Analysis of Organotypic Slices
- 08:55Images of Electroporated Neurons
- 09:35Conclusion
At foretage en hurtig vurdering af funktionen af gener i udviklingen af cerebral cortex, beskriver vi fremgangsmåder, der involverer<em> Ex vivo</em> Elektroporering af plasmider co-udtrykkende inhiberende RNA (RNAi) og GFP i murin embryonisk cortex. Denne protokol er egnet til studiet af forskellige aspekter af nervesystemets udvikling, f.eks neurogenese, neuronal migration og neuronal morfogenese herunder dendritceller og axon udvækst.
Tags
Neuronal MorphogenesisEx Vivo RNAi ElectroporationEmbryonic Murine Cerebral CortexCerebral CortexHigher Cognitive FunctionsSix Layered StructureInside-first Outside-last MannerNeuronal MigrationRegulation Of Neuronal MorphogenesisPrimary CulturesOrganotypic SlicesPSilencer Modified VectorU6 PromoterDouble Stranded Hairpin RNACMV PromoterGFP ProteinNeurite OutgrowthCandidate GenesMosaic Analysis
