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Bioengineering
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网站特定的细菌染色体工程:ΦC31整合介导的盒式交换(IMCE)
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JoVE Journal
Bioengineering
Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)
Please note that all translations are automatically generated.
Click here for the English version.
网站特定的细菌染色体工程:ΦC31整合介导的盒式交换(IMCE)
DOI:
10.3791/3698-v
•
08:21 min
•
March 16, 2012
•
John R. Heil
,
Jiujun Cheng
,
Trevor C. Charles
1
Biology
,
University of Waterloo
Chapters
00:05
Title
01:46
Production of Culture
02:53
Culture Preparation and Mixing (Mating)
04:27
Isolation of Trans-integrants
05:55
Results of Integrase Mediated Cassette Exchange (IMCE)
08:01
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
描述一个快速和有效的方法融入预制受体菌株,称为停机坪株,外源DNA的利益。该方法允许进入工程着陆垫一个给定的应变轨迹的站点特定的DNA盒的整合,通过共轭ΦC31整合表达。
Tags
Site-specific
Bacterial Chromosome Engineering
ΦC31 Integrase
Cassette Exchange
AttB
AttP
Recombination
Landing Pad
Spectinomycin-resistance Gene
AadA
E. Coli ß-glucuronidase Gene
UidA
Sinorhizobium Meliloti
Ochrobactrum Anthropi
Agrobacterium Tumefaciens
AmpC Locus
TetA Locus
Mobilizable Donor Vectors
Stuffer Red Fluorescent Protein (rfp) Gene
Antibiotic Resistance Gene
Gentamicin Resistant Plasmid PJH110
SphI
PstI
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