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Chapters
- 00:05Title
- 01:20Culturing Human Embryonic Stem Cells
- 02:05Definitive Endoderm and Pancreatic Progenitor Induction
- 03:01Pancreatic Maturation
- 03:57qRT-PCR Analysis
- 05:06Immunocytochemistry
- 06:21Analysis of Differentiation of Human Embryonic Stem Cells into Insulin-producing Cells
- 08:07Conclusion
De huidige studie beschrijft een gerichte aanpak van differentiatie in het induceren van de alvleesklier differentiatie van humane embryonale stamcellen. Van grote betekenis is de bevinding dat endotheelcel co-cultuur bemiddelt rijping van menselijke embryonale stamcellen afkomstig van de pancreas voorlopercellen in insuline tot expressie brengen.
Tags
Endothelial Cell Co-cultureHuman Embryonic Stem CellsDirected Differentiation ApproachPancreatic Insulin Producing CellsEmbryonic Stem CellsPluripotentCell Based TherapyRegenerative TreatmentCellular DifferentiationOrganogenesisPancreas DevelopmentEndoderm InductionNodal Pathway ModulationActivin AGrowth FactorsSonic Hedgehog InhibitionCyclopaminePancreatic MaturationNotch Signaling InhibitionVascularization
