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Chapters
- 00:05Title
- 01:20Culturing Human Embryonic Stem Cells
- 02:05Definitive Endoderm and Pancreatic Progenitor Induction
- 03:01Pancreatic Maturation
- 03:57qRT-PCR Analysis
- 05:06Immunocytochemistry
- 06:21Analysis of Differentiation of Human Embryonic Stem Cells into Insulin-producing Cells
- 08:07Conclusion
Den aktuella studien beskriver en riktad differentiering strategi att framkalla bukspottkörteln differentiering av mänskliga embryonala stamceller. Av stor betydelse är upptäckten att endotelcell samodling förmedlar mognad av mänskliga embryonala stamceller som härrör pankreas stamceller till insulinproducerande celler som uttrycker.
Tags
Endothelial Cell Co-cultureHuman Embryonic Stem CellsDirected Differentiation ApproachPancreatic Insulin Producing CellsEmbryonic Stem CellsPluripotentCell Based TherapyRegenerative TreatmentCellular DifferentiationOrganogenesisPancreas DevelopmentEndoderm InductionNodal Pathway ModulationActivin AGrowth FactorsSonic Hedgehog InhibitionCyclopaminePancreatic MaturationNotch Signaling InhibitionVascularization
