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配对末端标签测序(嘉PET)测绘染色质相互作用和了解转录调控染色质相互作用分析
JoVE Journal
Biology
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JoVE Journal
Biology
Chromatin Interaction Analysis with Paired-End Tag Sequencing (ChIA-PET) for Mapping Chromatin Interactions and Understanding Transcription Regulation
Please note that all translations are automatically generated.
Click here for the English version.
配对末端标签测序(嘉PET)测绘染色质相互作用和了解转录调控染色质相互作用分析
DOI:
10.3791/3770-v
•
21:55 min
•
April 30, 2012
•
Yufen Goh*
1
,
Melissa J. Fullwood*
1,2,3
,
Huay Mei Poh
,
Su Qin Peh
,
Chin Thing Ong
,
Jingyao Zhang
,
Xiaoan Ruan
,
Yijun Ruan
3
1
Genome Institute of Singapore
,
Agency for Science, Technology and Research, Singapore
,
2
A*STAR-Duke-NUS Neuroscience Research Partnership, Singapore
,
3
Department of Biochemistry
,
National University of Singapore, Singapore
Chapters
00:02
Title
01:00
Overall View of Chromatin Interaction Analysis using Paired-end Tag (ChIA-PET)
02:15
ChIP – Chromatin Immunoprecipitation
04:24
Cell Lysis
05:07
Nuclear Lysis
07:11
Fragmentation of Chromatin
08:26
Washing, Preclearing, and Coating of Antibody to Beads
10:08
Chromatin Immunopreciptation
11:20
Washing and Elution
11:48
ChIA-PET – Chromatin Interaction Analysis using Paired-end Tag Sequencing
12:29
End-blunting of ChIP DNA Fragments
13:33
Ligation of Biotinylated Half-linkers to ChIP DNA
16:02
Elution and Proximity Ligation of ChIP DNA Fragments
18:08
Immobilization of ChIA-PET DNA to Streptavidin Beads
20:47
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
配对末端标签测序(嘉PET)的染色质相互作用分析方法是<em>从头</em>染色质相互作用的检测,为更好地理解转录调控。
Tags
Chromatin Interaction Analysis
Paired-End Tag Sequencing
ChIA-PET
Mapping Chromatin Interactions
Transcription Regulation
Three-dimensional Structures
Higher-order Conformations
Nuclear Spaces
Gene Promoters
Regulatory Elements
Transcription Factors
Regulatory Sequences
Network Of Transcription Regulation
Coordination
Chromatin Immunoprecipitation
ChIP
Chromatin Fragments
Proximity Ligation
Covalent Links
DNA Fragments
Chromatin Complexes
MmeI Restriction Enzyme Sites
Half-linkers
PET Constructs
Article
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