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En genetisk skærm for at isolere Toxoplasma gondii Host-celle afstigningstrin Mutants
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Chapters
- 00:05Title
- 01:39Titration of Mutagen
- 05:59Enrichment of Egress Mutants
- 08:48Validation of Mutant Phenotypes by Egress Assays
- 10:21Cloning of Egress Mutants by Limiting Dilution
- 12:41Representative Data
- 14:14Conclusion
Forward genetik er en stærk metode til at optrævle det molekylære niveau af, hvordan<em> Toxoplasma</em> Egresses fra værtscellen. Protokoller er tilvejebragt til kemisk mutagenisere parasitter, berige for mutanter med defekter i induceret udgang, og validere fænotypen af klonede mutanter.
Tags
Genetic ScreenToxoplasma GondiiHost-cell Egress MutantsObligate Intracellular ParasiteLytic Replication CycleActive InvasionReplication Inside Host CellActive EgressMolecular LevelSignaling PathwaysPharmacological AgentsTriggers Of EgressIntracellular Ca2+Candidate Gene ApproachPlant Like Calcium Dependent Protein Kinase (CDPK)(chemical) Genetic ApproachesScreen For Mutant ParasitesDefect In Host Cell EgressChemical MutagenesisN-ethyl-N-nitrosourea (ENU)
