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Una pantalla genética para aislar Toxoplasma gondii Célula huésped-mutantes Egress
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Chapters
- 00:05Title
- 01:39Titration of Mutagen
- 05:59Enrichment of Egress Mutants
- 08:48Validation of Mutant Phenotypes by Egress Assays
- 10:21Cloning of Egress Mutants by Limiting Dilution
- 12:41Representative Data
- 14:14Conclusion
Adelante la genética es un poderoso método para desentrañar el nivel molecular de cómo<em> Toxoplasma</em> Egresados de su célula huésped. Protocolos se proporcionan para mutagenizar químicamente parásitos, enriquecer a los mutantes con defectos en la salida inducida, y validar el fenotipo de los mutantes clonados.
Tags
Genetic ScreenToxoplasma GondiiHost-cell Egress MutantsObligate Intracellular ParasiteLytic Replication CycleActive InvasionReplication Inside Host CellActive EgressMolecular LevelSignaling PathwaysPharmacological AgentsTriggers Of EgressIntracellular Ca2+Candidate Gene ApproachPlant Like Calcium Dependent Protein Kinase (CDPK)(chemical) Genetic ApproachesScreen For Mutant ParasitesDefect In Host Cell EgressChemical MutagenesisN-ethyl-N-nitrosourea (ENU)
