A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:34Preparation of Mouse Embryonic Fibroblasts (MEF) Feeders
- 02:21Co-seeding hESCS onto MEFs
- 03:55Depleting MEFs from the Co-culture
- 04:50Results: hESCs Purified from Co-culture
- 06:40Conclusion
Til tross for pågående arbeidet med å overgangen kulturer til mater-frie forhold, avledning og kultur av menneskelige embryonale stamceller (hESC) fortsatt i stor grad avhengig av co-kulturer med mus embryonale feeders (MEFs). Her viser vi en ny metodikk for raskt å fjerne feeders fra hESC kulturer før eksperimentering.
Tags
Fibroblast RemovalHigh Density Human Embryonic Stem Cell CulturesMouse Embryonic FibroblastsFeeder SystemHESC PurityModified Culture SystemsMitomycin C-treated Mouse Embryonic FibroblastsDifferentiation ExperimentsPurification TechniquesFACSMACSDrug Resistant VectorsPurer Population Of HESCsAspiration Of MEF Sheet
