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一个从人体细胞分泌蛋白生产的方便,一般的表达平台
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Biology
A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells
Please note that all translations are automatically generated.
Click here for the English version.
一个从人体细胞分泌蛋白生产的方便,一般的表达平台
DOI:
10.3791/4041-v
•
07:09 min
•
July 31, 2012
•
Halil Aydin*
1
,
Farshad C. Azimi*
1
,
Jonathan D. Cook*
1
,
Jeffrey E. Lee
1
Department of Laboratory Medicine and Pathobiology
,
University of Toronto
Chapters
00:05
Title
01:41
Small-scale Test Expression
03:34
Large-scale Expression and Purification
05:44
Results: Human Cell Surface Proteins Purified from Large Scale HEK 293T Cells
06:31
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在人类后基因组时代,在本机构的重组蛋白的结构,功能和治疗的研究和发展是至关重要的。在这里,我们描述了测试和大规模的蛋白质在人类胚胎肾细胞293T,可用于生产各种重组蛋白表达系统。
Tags
Convenient Expression Platform
Secreted Proteins
Recombinant Protein Expression
Human Cells
Bacteria
E. Coli
Prokaryotic Hosts
Human Proteins
Yeast Expression System
Baculovirus-infected Insect Cells
Cell-free In Vitro Translation System
Mammalian Host
Mammalian Cell Lines
HEK 293 Cells
CV-1 Cells
CHO Cells
Protein Yields
Cost-effective Production
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