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細菌の凍結乾燥と細胞の生存への影響のための処方
JoVE Journal
Biology
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JoVE Journal Biology
Formulations for Freeze-drying of Bacteria and Their Influence on Cell Survival
DOI:

08:55 min

August 03, 2013

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Chapters

  • 00:05Title
  • 00:46Cultivation and Harvest of P. putida
  • 01:37Formulation Solutions, Preparing Cells for Freeze-drying, and Surface Tension Measurements
  • 03:22Characterization of Formulation Freezing Behavior by Differential Scanning Calorimetry (DSC) and Freeze-drying
  • 05:31X-ray Analysis of the Dry Formulations and Electron Microscopy
  • 06:38Representative Freeze-drying Results
  • 08:37Conclusion

Summary

Automatic Translation

凍結乾燥は、多くの場合、生菌細胞の乾燥製品を得るための簡単​​で便利な方法です。プロセスの問題は、細胞生存ある。ここで我々は細部、凍結乾燥時の細胞の生存が使用製剤の特性によって影響されるか調査する手順を。

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