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1Centre for Medical Parasitology, Department of International Health, Immunology & Microbiology, Faculty of Health Sciences,University of Copenhagen, 2Department of Infectious Diseases,Copenhagen University Hospital (Rigshospitalet), 3Institute of Infection and Immunology Research, School of Biology,University of Edinburgh
Chapters
- 00:00Title
- 02:00Day 1
- 02:43Day 2
- 02:58Reagents and Materials
- 04:14Thin Smears and Fixation of Parasites Prior to in situ Hybridization
- 07:06Hybridization of RNA Probes to Fixed Slides
- 08:51Antibody Conjugation & Fluorescence Amplification
- 11:12Visualization of Hybridized Probes
- 11:27Representative Results & Discussion
Fluorescente<em> In situ</em> Hibridização (FISH) para identificar transcritos de ARNm em células individuais permite a análise da actividade poligênica tais como a transcrição simultânea de mais do que um membro da<em> Var</em> Família multigênica em<em> Plasmodium falciparum</em> Eritrócitos infectados<sup> 1</sup>. A técnica é adaptável e pode ser usado em diferentes tipos de genes, células e organismos.
Tags
Single-cell Gene TranscriptionRNA Fluorescent In Situ Hybridization (FISH)Plasmodium Falciparum Infected ErythrocytesPfEMP1 Protein VariantsVar GenesMutually Exclusive RegulationTranscription AssaysRNA-FISH MethodologyDigoxigenin-labeled Antisense RNA ProbesBiotin-labeled Antisense RNA ProbesTSA Plus Fluorescence Palette System
