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Neuroscience
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从小鼠胚胎神经管的神经嵴细胞的分离和培养
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Neuroscience
Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube
Please note that all translations are automatically generated.
Click here for the English version.
从小鼠胚胎神经管的神经嵴细胞的分离和培养
DOI:
10.3791/4134-v
•
12:48 min
•
June 02, 2012
•
Elise R. Pfaltzgraff
,
Nathan A. Mundell
2
,
Patricia A. Labosky
1
Department of Cell and Developmental Biology, Center for Stem Cell Biology
,
Vanderbilt University Medical Center
,
2
Department of Pharmacology, Center for Stem Cell Biology
,
Vanderbilt University Medical Center
,
3
Vanderbilt University Medical Center
Chapters
00:05
Title
01:14
Introduction
01:36
Prepare Plates
02:18
Preparation of Self Renewal and Wash Media
03:06
Preparation of Collagenase/Dispase
04:22
Isolating Vagal and Trunk Neural Tube
08:21
Removal of Non-Neural Ectoderm and Mesoderm
11:06
Removal of the Neural Tube
11:46
Representative Results
12:35
Conclusions
Summary
Automatic Translation
English (Original)
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Automatic Translation
胚胎神经嵴的神经管分离,便于使用<em>在体外</em>学习迁移,自我更新和多能神经嵴的方法。
Tags
Neural Crest Cells
Embryonic Murine Neural Tube
Isolation
Culture
Multipotent Progenitor Population
Epithelial To Mesenchymal Transition
Migration
Self-renewal
Avian Neural Tube
Rat Systems
Explanted NC Cells
Proliferation
Transplantation
Cellular Properties
Neural Tube Explant Assay
Mammalian NC
Isolation Methods
Post-migratory NC Progenitors
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