Journal
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胎児マウス神経管から神経堤細胞の単離および培養
JoVE Journal
Neuroscience
Author Produced
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JoVE Journal Neuroscience
Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube
DOI:

12:48 min

June 02, 2012

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Chapters

  • 00:05Title
  • 01:14Introduction
  • 01:36Prepare Plates
  • 02:18Preparation of Self Renewal and Wash Media
  • 03:06Preparation of Collagenase/Dispase
  • 04:22Isolating Vagal and Trunk Neural Tube
  • 08:21Removal of Non-Neural Ectoderm and Mesoderm
  • 11:06Removal of the Neural Tube
  • 11:46Representative Results
  • 12:35Conclusions

Summary

Automatic Translation

神経管から胚の神経堤の分離の使用を容易に<em> in vitroで</em>移行、自己再生し、神経堤の多能性を研究するための方法。

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