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Neuroscience
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胎児マウス神経管から神経堤細胞の単離および培養
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube
Please note that all translations are automatically generated.
Click here for the English version.
胎児マウス神経管から神経堤細胞の単離および培養
DOI:
10.3791/4134-v
•
12:48 min
•
June 02, 2012
•
Elise R. Pfaltzgraff
,
Nathan A. Mundell
2
,
Patricia A. Labosky
1
Department of Cell and Developmental Biology, Center for Stem Cell Biology
,
Vanderbilt University Medical Center
,
2
Department of Pharmacology, Center for Stem Cell Biology
,
Vanderbilt University Medical Center
,
3
Vanderbilt University Medical Center
Chapters
00:05
Title
01:14
Introduction
01:36
Prepare Plates
02:18
Preparation of Self Renewal and Wash Media
03:06
Preparation of Collagenase/Dispase
04:22
Isolating Vagal and Trunk Neural Tube
08:21
Removal of Non-Neural Ectoderm and Mesoderm
11:06
Removal of the Neural Tube
11:46
Representative Results
12:35
Conclusions
Summary
Automatic Translation
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Automatic Translation
神経管から胚の神経堤の分離の使用を容易に<em> in vitroで</em>移行、自己再生し、神経堤の多能性を研究するための方法。
Tags
Neural Crest Cells
Embryonic Murine Neural Tube
Isolation
Culture
Multipotent Progenitor Population
Epithelial To Mesenchymal Transition
Migration
Self-renewal
Avian Neural Tube
Rat Systems
Explanted NC Cells
Proliferation
Transplantation
Cellular Properties
Neural Tube Explant Assay
Mammalian NC
Isolation Methods
Post-migratory NC Progenitors
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